Isolation, characterization and molecular weight determination of collagen from marine sponge Spirastrella inconstans (Dendy)

  • S Sudharsan
  • P Seedevi
  • R Saravanan
  • P Ramasamy
  • S Vasanth Kumar
  • S Vairamani
  • A Srinivasan
  • A Shanmugam

Abstract

Collagen is a major structural protein of connective tissues. It can be used as a prosthetic biomaterial applicable to artificial skin, tendon ligaments and development collagen implants. In the present study, an attempt was made to isolate and characterize collagen from the marine sponge, Spirastrella inconstans. The total protein content of sponge collagen was relatively high (32%). While determining the molecular weight of crude and purified collagen through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the crude showed three bands (80, 60 and 59 kDa molecular weight) and purified showed only a single band (58 kDa). The structural properties were analyzed by using fourier transform infra red (FT-IR) spectrum and the stability of collagen was also given the single transition peak in differential scanning calorimetry (DSC). The microstructure of sponge collagen showed highly porous and interconnected scaffolds in scanning electron microscopic (SEM) analysis.

Keywords: Collagen, Spirastrella inconstans, SDS-PAGE, Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM)

African Journal of Biotechnology Vol. 12(5), pp. 504-511

Author Biographies

S Sudharsan
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, Tamil Nadu, India
P Seedevi
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, Tamil Nadu, India
R Saravanan
Department of Marine Pharmacology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Kelambakkam-603 103, Tamil Nadu, India
P Ramasamy
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, Tamil Nadu, India
S Vasanth Kumar
Department of Chemical Engineering, Annamalai University, Annamalai Nagar- 608002, Tamil Nadu, India
S Vairamani
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, Tamil Nadu, India
A Srinivasan
Department of Biophysics, All India Institute of Medical Sciences, New Delhi-110 029, India
A Shanmugam
Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai-608 502, Tamil Nadu, India
Published
2015-11-29
Section
Articles

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eISSN: 1684-5315