African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register

Optimization of cholesterol oxidase production by Brevibacterium sp. employing response surface methodology

Shengli Yang, Hui Zhang


An ultrasound-assisted emulsification as a pretreatment for cholesterol oxidase production by submerge fermentation using Brevibacterium sp. in a batch system was studied. Medium improvement for the production employing response surface methodology (RSM) was optimized in this paper. The concentration of Tween-80, cholesterol and the time of ultrasonic pretreatment medium were further optimized by RSM. Results from RSM showed that the initial concentration of cholesterol, Tween-80 and pretreatment time exerted a significant effect on cholesterol oxidase production, but these factors did not exert a significant effect on cell growth. The improved medium consisted of 4.076 g/L cholesterol, 0.2932‰ (v/v) Tween-80, 22.361 (min) treatment time, and cholesterol oxidase production reached 1.483 U/ml after 36 h culture, which was 83.57% greater than the control medium.

Keywords: Ultrasonic, cholesterol oxidase, response surface methodology
AJOL African Journals Online