Induction of apoptosis in human breast adenocarcinoma MCF-7 cells by tannic acid and resveratrol

  • Ahu Soyocak
  • Didem Turgut Cosan
  • Ayse Basaran
  • Hasan Veysi Gunes
  • Irfan Degirmenci
  • Fezan Sahin Mutlu

Abstract

Tannic acid (TA), a plant polyphenol, has been described as having anticarcinogenic, antioxidant, antimutagenic, antimicrobial, antiallergic, antiinflammatory and astringent properties. Resveratrol (RES), a phytoalexin phenolic compound, has been known as an antioxidant, anticarcinogenic, antiinflammatory, antimutagenic, antiproliferative, antiviral, antibacterial, estrogenic and vasodilator agent. Although molecular mechanisms of TA and RES responsible for anticancer activity are yet to be known, there are in vitro studies indicating that these substances contribute to anticancer activity by inducing apoptosis. In this study, the effects of TA and RES in MCF-7 breast cancer cells on apoptotic index, FAS-associated death domain (FADD) and Bak proteins were investigated. Terminal transferase-mediated dUTP nick end-labeling (TUNEL) method was applied for apoptosis and immunohistochemical staining for FADD and Bak proteins. Our study results show that TA and RES increased apoptotic index, percentage ratios of apoptotic proteins Bak and FADD in MCF-7 cell line.

Keywords: Bak, Fas-associated death domain (FADD), MCF-7, resveratrol, tannic acid

African Journal of Biotechnology Vol. 12(12), pp. 1431-1437

Author Biographies

Ahu Soyocak
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
Didem Turgut Cosan
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
Ayse Basaran
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
Hasan Veysi Gunes
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
Irfan Degirmenci
Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
Fezan Sahin Mutlu
Department of Biostatistics and Medical Informatics, Medical Faculty, Eskisehir Osmangazi University,
26480 Eskisehir, Turkey
Published
2016-01-19
Section
Articles

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eISSN: 1684-5315