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African Journal of Biotechnology

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Rubisco-bis-phosphate oxygenase (RuBP)- A potential housekeeping gene for qPCR assays in tea

Bornali Gohain, Tirthankar Bandyopadhyay, Priyadarshini Bhorali, Sangeeta Borchetia, Raju Bharalee, Sushmita Gupta, Niraj Agarwala, Neelakshi Bhattacharyya, Ranjit Singh, Prasenjit Bhagawati, Parveen Ahmed, MC Kalita, Sudripta Das

Abstract


The present experiment is an effort to find a stable reference gene in Camellia sinensis and Camellia assamica under different biotic and abiotic stresses. This study evaluate the variation in gene expression across tea leaf tissues in nine experiments. The suitability of 18S rRNA, 26S rRNA, rubiscobis- phosphatase oxygenase (RuBP) and Camellia tubulin (CaT) as reference genes were validated by geNorm and BestKeeper programs. The finding reveals 18S rRNA and RuBP to be the most stably expressed housekeeping genes, the latter being the first report of its kind in tea. The finding paves the way for their application in accurate quantification of trait specific gene expression and other genomic studies in tea.

Keywords: Camellia sinensis, Camellia assamica, qPCR, BestKeeper, geNorm, housekeeping gene




http://dx.doi.org/10.5897/AJB11.1737
AJOL African Journals Online