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African Journal of Biotechnology

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Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies

Anita Rawat, Santan Barthwal, HS Ginwal

Abstract


A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted DNA ranged from 6.98 to 19.668 μg / 100 mg tissue and A260 / A280 ratio ranged from 1.70 to 1.87. The polymerase chain reaction (PCR) amplification of the DNA extracted from sapwood tissue using random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers was similar to that of DNA extracted from the needle tissues.

Keywords: Pinus roxburghii, DNA extraction, sapwood, random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR)

African Journal of Biotechnology Vol. 12(15), pp. 1732-1735



http://dx.doi.org/10.5897/AJB12.2733
AJOL African Journals Online