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African Journal of Biotechnology

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Characterization of specific random amplified polymorphic (RAPD) DNA fragments related to catechin content for early detection methods in gambier plant (Uncaria gambir (Hunter) Roxb.)

Istino Ferita, Hamda Fauza, Irfan Suliansyah, Gustian Gustian, Jamsari Jamsari

Abstract


The existence of a target DNA fragments associated with the potential high level of catechins in gambier plant was carried out preliminary using RAPD markers. Target fragment was ligated and transformed into Escherichia coli DH5α strains using pGem-T Easy vector. The purpose of this study was to obtain information of specific DNA fragments and to generate specific primer combinations associated with potentially high content of catechins. DNA sequence was successfully generated with 206 nucleotides in length. The combination of specific primers, designated as Udtg3 (5'-CAGATCCTTTGCCACACTGA-3') and Udtg4 (5'- CGTACCTGCCAA CATA ACAG - 3') were successfully designed and could be applied as a diagnostic marker in detection of catechin content production of gambier plant.

Keywords: Gambier, RAPD, specific primers, MAS, catechin

African Journal of Biotechnology Vol. 12(15), pp. 1736-1744



http://dx.doi.org/10.5897/AJB12.2814
AJOL African Journals Online