African Journal of Biotechnology

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Molecular markers for genetic diversity and phylogeny research of Brazilian sheep breeds

Bruno do Amaral Crispim, Márcia Cristina Matos, Leonardo de Oliveira Seno, Alexéia Barufatti Grisolia


Brazilian sheep descended from several breeds brought to the New World by Portuguese and Spanish colonists, and they have evolved and adapted to local climatic variations and acquired tolerance or resistance to many diseases. Molecular markers are widely used in analyzing genetic variability, and markers such as amplified fragment length polymorphism (AFLP), restriction fragment length polymorphism (RFLP), microsatellite, mtDNA and single nucleotide polymorphism (SNP) have facilitated the characterization of genetic diversity and population structure, and in the investigation of the natural history, behavior, and evolution of several sheep breeds. In this context, we present here a review of the uses of molecular markers in ecological and conservation research of Brazilian sheep breeds.

Key words: DNA polymorphism, genetic conservation, Ovis aries.
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