Development and evaluation of a plant-based air filter system for bacterial growth control

  • Yong-Keun Choi
  • Seongmin Lee
  • Hyeon Jin Jeon
  • Heehoon Jung
  • Hyung Sup Kim
  • Kil Koang Kwon
  • Su Mi Kim
  • Kyung-Guen Song
  • Im Soon Lee
  • Byung Uk Lee
  • Hyung Joo Kim

Abstract

We investigated a novel plant-based air filter system for bacterial growth control. The volatile components released from the experimental plant (Cupressus macrocarpa) were used as the basis of the bacterial growth control and inhibition. We monitored the effect of light on the gas exhausted from the system, and we found that the presence of light induced an increase in the O2 concentration and a decrease in the CO2 concentration in the exhaust gas. A variety of Gram-positive and -negative bacteria was used to elucidate the effect of the exhaust gas on bacterial growth. In the Bacillus subtilis cultivation aerated with the exhaust gas (batch mode), we observed a decrease in the specific growth rate (μ = 0.227 h-1) compared with the control experiments (0.257 h-1). The same result was observed for the Staphylococcus aureus cultivation aerated with the exhaust gas. In the case of Gram-negative bacterial cultivation aerated with the gas, no significant inhibitory effect of the exhaust gas on the bacterial growth was observed. When the number of bacteria (B. subtilis) in a continuous culture was varied at different aeration rates (between 50 to 200 mL/min) using the exhaust gas, a prominent inhibitory effect was observed. Preliminary gas analysis showed that the major inhibitory factors in the exhaust gas are α- and β-pinene and linalool. The results show that the air filter system used in this study could be applied not only as a methodological aspect for estimating antibacterial activity but also for bacteria control in a given system.

Keywords: Plant-based biofilter, Cupressus macrocarpa, Bacillus subtilis, Staphylococcus aureus, α-pinene, β-pinene

African Journal of Biotechnology Vol. 12(16), pp. 2027-2033

Author Biographies

Yong-Keun Choi
Department of Microbial Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Seongmin Lee
Department of Microbial Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Hyeon Jin Jeon
Department of Microbial Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Heehoon Jung
Department of Microbial Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Hyung Sup Kim
Department of Textile Engineering, Konkuk University, 1 Hwayang-dong, Gwangjing-gu, Seoul, South Korea
Kil Koang Kwon
Department of Microbial Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Su Mi Kim
School of Cosmetology, Kyungbok University, 154 Sinbuk-Myeon, Pocheon, Gueonggi, South Korea
Kyung-Guen Song
Water Research Center, Korea Institute of Science and Technology, Cheongryang, Seoul, South Korea.
Im Soon Lee
Department of Biological sciences, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Byung Uk Lee
Department of Mechanical Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Hyung Joo Kim
Department of Microbial Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, South Korea.
Published
2016-01-29
Section
Articles

Journal Identifiers


eISSN: 1684-5315