Effect of zeatin on the infection process and expression of MAPK-4 during pathogenesis of Alternaria brassicae in non-host and host Brassica plants

  • K Kumar Marmath
  • P Giri
  • G Taj
  • D Pandey
  • A Kumar

Abstract

Recent studies have revealed an important role of hormones in plant immunity. Cytokinins are phytohormones that are involved in various regulatory processes including plant defense. Zeatin a cytokinin antagonizes the effect of Alternaria brassicae pathotoxin in cell culture of Brassica juncea. Phytohormones are also the inducers of MAP Kinase signaling pathways which are the important signaling modules in eukaryotic cells. In this paper, an attempt was made to study the exogenous application of zeatin on the disease score, infection behavior of A. brassicae, expression pattern of MAPK-4 in the non host Sinapsis alba, host B. juncea susceptible c.v Varuna, B. juncea moderately tolerant c.v. Divya and transgenic Brassica to confer its role in plant immunity. We observed that high concentration of zeatin led to increased defense responses by delaying the infection process as well as significantly reducing the disease score. Semi-quantitative RT-PCR reveals that zeatin also increases the expression of MAPK-4 at early hours of infection. Our result supports that zeatin up regulates plant immunity via an elevation of MAPK-4 and clearly reflects that it antagonizes the effect of A. brassicae. The crosstalk between zeatin and MAPK signaling pathway may help plants fine-tune defense responses against A. brassicae in the disease Alternaria blight.

Keywords: Alternaria blight, Brassica, disease score, MAPK, pathogenesis, infection process

African Journal of BiotechnologyVol. 12(17), pp. 2164-2174

Author Biographies

K Kumar Marmath
Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Dist. US Nagar, Uttarakhand, India
P Giri
Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Dist. US Nagar, Uttarakhand, India
G Taj
Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Dist. US Nagar, Uttarakhand, India
D Pandey
Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Dist. US Nagar, Uttarakhand, India
A Kumar
Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Dist. US Nagar, Uttarakhand, India
Published
2016-01-29
Section
Articles

Journal Identifiers


eISSN: 1684-5315