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African Journal of Biotechnology

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Identification of RAPD markers linked to salinity tolerance in wheat

Waqas Manzoor Bhutta, Muhammad Hanif

Abstract


Genetic diversity can be measured by a number of ways, including pedigree, phenotype and allelic diversity at loci controlling phenotypes of interest. A DNA marker for root length in wheat (Triticum aestivum L.) was identified. The individual plants from F2 population segregation for salinity tolerance and the parents (S-24 and DN-27) were grown in polyethylene tubes under saline conditions (EC 16 dS m-1) for root length at the four leaf stage. The plants were then transplanted into pots supplied with optimum water and nutrients until maturity. Genomic DNA from 50 tolerant and 50 sensitive F2 plants was extracted. The bulked segregants analysis was used in the random amplified polymorphic DNA (RAPD) technique. DNA polymorphisms were observed using 240 primers. The primer, GLE-14 amplified a 970 bp polymorphic DNA fragment and this DNA fragment can be used for marker-assisted selection to breed for salt tolerant wheat. This marker should be further used while selecting different crops under salt stress.

Keywords: Marker, RAPD, root length, salinity, wheat

African Journal of Biotechnology Vol. 12(17), pp. 2175-2181



http://dx.doi.org/10.5897/AJB09.973
AJOL African Journals Online