Molecular studies on some soil-Streptomyces strains of western region, Kingdom of Saudi Arabia (KSA)

  • SH Mohamed
  • BO Shori Ghadeer
  • AS Sadik

Abstract

Random amplified polymorphic of DNA-polymerase chain reaction (RAPD-PCR) analysis of the DNA extracted from seven Streptomyces strains of western region, KSA was the aim of this study. Partial sequence of 16S rRNA gene of Streptomyces polychromogenes was also attempted. Results show that a total number of 240 amplified fragments were amplified using 10 RAPD-PCR primers (OPA11, OPB10, OPB15, OPC03, OPC07, OPC18, OPD05, OPE05, OPO14 and OPO17). A total number of 97, 73, 88, 79, 100, 108 and 82 fragments were amplified from the DNA extracts of S. polychromogenes, Streptomyces chattanoogensis, Streptomyces lucensis, Streptomyces antibioticus, Streptomyces violans, Streptomyces griseorubiginosus and Streptomyces violaceus, respectively. An obvious variation in the amplified fragments was recorded using the 10 RAPD-PCR primers (31, 27, 17, 19, 23, 29, 24, 26, 23 and 21 fragments for the primers, respectively). The highest similarity (66.7%) was found between S. lucensis and S. chattanoogensis; lowest similarity (35%) was recorded between the gray S. chattanoogensis and the red S. violaceus. The 16S rRNA gene was isolated via PCR from the DNA of S. polychromogenes (1) and sequenced. Fragments of 1003 and 837 nts were amplified using the forward and reverse primers, respectively. On matching, a final sequence of about 1452 nts (GenBank: JQ962978.1) was obtained and compared with five universal Streptomyces strains and four bacterial clones. The percent identities between the isolate of this study and the compared bacterial strains was lowest (79.1%) compared to HQ844464.1 and highest (98.3%) compared to EU520331.1. Based on the phenotypic and genotypic (16S rRNA gene) features, the strain could be classified as a new strain of S. polychromogenes.

Keywords: Identification, Streptomyces, RAPD-PCR, 16S rRNA gene, KSA

African Journal of Biotechnology Vol. 12(19), pp. 2558-2564

Author Biographies

SH Mohamed
Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, Kingdom of Saudi Arabia; Soil, Water and Environmental Research Institute, Agricultural Research Center, 9 Gamaa st., P.O. Box 12619, Giza, Egypt
BO Shori Ghadeer
Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, Kingdom of Saudi Arabia
AS Sadik
Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, Kingdom of Saudi Arabia; Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, P.O. Box 68 Hadayek Shubra, 11241, Cairo, Egypt
Published
2016-02-06
Section
Articles

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eISSN: 1684-5315