Technological and cost comparison of cytochrome P450 2B6 (516G>T) genotyping methods in routine clinical practice

  • Milcah Dhoro
  • Charles Nhachi
  • Collen Masimirembwa

Abstract

Pharmacogenetics requires robust and affordable tests to determine genetic variability. This study compares three genotyping methods: gene re-sequencing, real time polymerase chain reaction (PCR) allelic discrimination and PCR-RFLP for the detection of a genetic variation (516G>T) in the gene which codes for the enzyme, CYP2B6, the main enzyme in the metabolic pathway of the antiretroviral drug, efavirenz. The CYP2B6 (516G>T) variant has reduced metabolic capacity. Twenty (20) samples obtained from human immunodeficiency virus acquired immunodeficiency syndrome (HIV/AIDS) positive patients on an efavirenz containing regimen were used to establish whether these methods produce the same CYP2B6 genotype results on the same samples. Results were directly compared for concordance and revealed a 100% correlation with all three methods. Comparison for cost of equipment and reagents required for each method revealed an order of: sequencing > real time-PCR > PCR-RFLP. This study demonstrates the reproducibility of these three methods and provides an opportunity for the clinical applicability in routine clinical practice.

Keywords: Polymorphism, drug response, efavirenz, Zimbabwe.

African Journal of Biotechnology Vol. 12(19), pp. 2706-2710

Author Biographies

Milcah Dhoro
African Institute of Biomedical Science and Technology, P.O. Box 2294, Harare, Zimbabwe; Department of Clinical Pharmacology, College of Health Sciences, University of Zimbabwe, Zimbabwe; Department of Molecular Sciences, African Institute of Biomedical Science and Technology, Dominion House, 211 Herbert Chitepo and Sam Nujoma Street, Harare, Zimbabwe
Charles Nhachi
Department of Clinical Pharmacology, College of Health Sciences, University of Zimbabwe, Zimbabwe.
Collen Masimirembwa
African Institute of Biomedical Science and Technology, P.O. Box 2294, Harare, Zimbabwe.
Published
2016-02-07
Section
Articles

Journal Identifiers


eISSN: 1684-5315