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African Journal of Biotechnology

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Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

Utpala Parthasarathy, O. P. Nandakishore, K. Nirmal Babu, Senthil Kumar, V. A. Parthasarathy

Abstract


A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia. ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia. ISSR markers showed clear distinction among the species whereas RAPD markers showed segregation based on geographical location as well as species based.

Key words: Garcinia, genetic diversity, inter-simple sequence repeats, randomly amplified polymorphic DNA, principal component analysis.




http://dx.doi.org/10.5897/AJB2013.13053
AJOL African Journals Online