DsRed gene expression by doxycycline in porcine fibroblasts and cloned embryos using transposon

  • SuJin Kim
  • JoonHo Moon
  • BegoRoibas da Torre
  • Islam M Saadeldin
  • JungTaek Kang
  • JiYei Choi
  • SolJi Park
  • Byeong-Chun Lee
  • Goo Jang Goo Jang

Abstract

To develop a transgenic pig, introduction of foreign genes into fibroblasts is required. In this study, Piggybac transposition was used to produce tetracycline dependent gene expressing cloned embryos. Red fluorescence proteins (DsRed) combined with tetracycline promoter flanked transposon sequences were transfected into fetal fibroblasts, and the transfected cells were used as the donor for somatic cell nuclear transfer. Induction of DsRed expression was successfully controlled by doxycycline treatment in donor fibroblasts and early stage embryos. In conclusion, this study suggested that Piggybac transposition could deliver genes into cells or embryos for developing transgenic pig.

Keywords: Miniature pigs, transfection, Piggybac, somatic cell nuclear transfer (SCNT), RFP

African Journal of Biotechnology Vol. 12(21), pp. 3188-3190

Author Biographies

SuJin Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
JoonHo Moon
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
BegoRoibas da Torre
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
Islam M Saadeldin
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
JungTaek Kang
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
JiYei Choi
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
SolJi Park
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
Byeong-Chun Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
Goo Jang Goo Jang
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Research Institute of Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea; Emergence Center for Food-Medicine Personalized Therapy System, Advanced Institutes of Convergence Technology, Seoul National University, Gyeonggi-do, Korea
Published
2016-03-10
Section
Articles

Journal Identifiers


eISSN: 1684-5315