Comparative genetic diversity analysis of oat (Avena sativa L.) by microsatellite markers and morphological rainfed expressions

  • Yogesh Ruwali
  • JS Verma
  • Lalan Kumar

Abstract

Equivalence was appraised between phenotypic and molecular markers (ISSR) to analyze the genetic diversity of 20 high yielding genotypes representing different geographical zones of the world. A moderate range of genetic similarity (0.84 to 0.20) was observed on the basis of 20 inter-simple sequence repeats (ISSR) markers, where it was found high (0.995 – 0.204) on the basis of 7 primary morphological rainfed expression. Genotypes in morphological character based dendogram were clustered into their respective geographic groups, while a random grouping was observed in dendogram based on the ISSR markers. A negative correlation (r = -0.186) was found among morphological and molecular marker systems, but the latter was found effective in distinguishing the genotypes using specific band positions for them. The genotypic classification agreed closely with the grouping observed in ISSR based 3D analysis.

Keywords: Avena sativa, diversity, inter-simple sequence repeats (ISSR), morphology, oat, relationship

African Journal of Biotechnology Vol. 12(22), pp. 3414-3424

Author Biographies

Yogesh Ruwali
Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantnagar, Dist. U.S. Nagar, Uttarakhand, 263145, India
JS Verma
Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantnagar, Dist. U.S. Nagar, Uttarakhand, 263145, India
Lalan Kumar
Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, Dist. U.S. Nagar, Uttarakhand, 263145, India
Published
2016-03-15
Section
Articles

Journal Identifiers


eISSN: 1684-5315