Phylogenetic relationship among Kenyan sorghum germplasms based on aluminium tolerance

  • Reuben K Cheprot
  • Thomas K Matonyei
  • Beatrice A Were
  • O. George Dangasuk
  • Augostino O. Onkware
  • Evans O Ouma
  • Emily J. Too
  • J Liu
  • S Gudu
  • LV Kochian

Abstract

Eighty nine (89) sorghum lines sourced from various parts of Kenya were used to determine phylogenetic relationships based on 10 DNA fragments at AltSB loci with SbMATE, ORF9 and MITE primers. Nine lines of varying aluminium tolerance levels were selected to compare their SbMATE gene expression via the real-time PCR quantification of SbMATE gene expression. The sorghum line MSCR O2 expressed a thousandfold more SbMATEgene activity than the sensitive lines (MSCRM49, MSCRN84 and MSCRN61) under Al treatment. Analysis was done by agarose gel electrophoresis stained with ethidium bromide. The objective of this study was to assess the level of phylogenetic relationships among the Kenyan sorghum germplasms at a known Al tolerance locus. Hierarchical cluster analysis joined at 70% simple matching coefficient using average linkage similarity level produced nine groups in which 67 lines fell in three major clusters of 39, 15 and 13 lines each. The three Al tolerant lines MSCRO2, MSCRC1 and MSCRN60 were clustered together. Lines MSCRO2, MSCRC1 and MSCRN60, screened to be Al tolerant were genetically related at 70% average linkage similarity level and therefore recommend their further development as a food security measure in Kenya.

Keywords: Aluminium (Al) toxicity, Sorghum bicolor, Sorghum bicolor multi-drug, toxic extrusion compound (SbMATE) gene expression

African Journal of Biotechnology Vol. 12(22), pp. 3528-3536

Author Biographies

Reuben K Cheprot
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
Thomas K Matonyei
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
Beatrice A Were
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
O. George Dangasuk
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
Augostino O. Onkware
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenyakiboir@yahoo.com
Evans O Ouma
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
Emily J. Too
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
J Liu
USDA-ARS Plant, Soil and Nutrition laboratory, Cornell University, Ithaca - NY, USA.
S Gudu
Department of Biological Sciences, Chepkoilel University College - Moi University, P.O Box 1125 - 30100, Eldoret, Kenya
LV Kochian
USDA-ARS Plant, Soil and Nutrition laboratory, Cornell University, Ithaca - NY, USA.
Published
2016-03-15
Section
Articles

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eISSN: 1684-5315