Haematological evaluation of Wistar rats exposed to chronic doses of cadmium, mercury and combined cadmium and mercury

  • ASY Hounkpatin
  • PA Edorh
  • P Guédénon
  • CG Alimba
  • A Ogunkanmi
  • TV Dougnon
  • G Boni
  • KA Aissi
  • S Montcho
  • F Loko
  • N Ouazzani
  • L Mandi
  • M Boko
  • EE Creppy

Abstract

Cadmium and mercury present in the environment, cause blood disorders. This study was conducted to evaluate the influence of cadmium, mercury and their combination on hematological parameters of Wistar rats. For this purpose, two different doses of each metal and their combination were administered orally for 28 days to six groups of five rats each. Two groups (A and B) were respectively exposed to CdCl2 (0.25 and 2.5 mg/kg), two other groups (C and D) respectively received HgCl2 (0.12 and 1.2 mg/kg) and the last two groups (E and F) were respectively treated with the combination of these two metals: (0.25 mg/kg Cd + 0.12 mg/kg Hg) and (2.5 mg/kg Cd + 1.2 mg/kg Hg). The control group (G) received the same volume of distilled water. At the end of exposure, bodies of rats were weighed and the whole blood was collected by retro-orbital sinus method for analysis of hematological parameters. The results of this study show a significant decrease (p<0.05) in white blood cells (WBC) in the lot treated with the combination (0.25 mg/kg Cd + 012 mg/kg Hg) and also indicate a significant decrease (p<0.05) in WBC, red blood cells (RBC), hemoglobin concentration (HGB) and the mean corpuscular hemoglobin concentration (MCHC) with high levels of mercury (2.5 mg/kg) and the combination (2.5 mg/kg Cd + 1.2 mg/kg Hg). An increase in the number of platelet count (PLT) in all intoxicated lots was observed.

Keywords: Cadmium, mercury, hematology, blood parameters, rats

African Journal of BiotechnologyVol. 12(23), pp. 3731-3737

Author Biographies

ASY Hounkpatin
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
PA Edorh
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin; Departement of Biochemistry and Cellular Biology, University of Abomey-Calavi, 01BP 526 Cotonou, Benin
P Guédénon
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
CG Alimba
Department of Cell Biology and Genetics, Faculty of Science, University of Lagos, Nigeria
A Ogunkanmi
Department of Cell Biology and Genetics, Faculty of Science, University of Lagos, Nigeria
TV Dougnon
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
G Boni
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
KA Aissi
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
S Montcho
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED), University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
F Loko
Polytechnic School of Abomey-Calavi, Research Laboratory in Applied Biology, University of Abomey-Calavi, 01 BP 2009 Cotonou, Benin.
N Ouazzani
Laboratory of Hydrobiology, Ecotoxicology and Cleansing, Department of Biology, Faculty of Science Semlalia, University Cadi Ayyad, BP 2390, Marrakech, Morocco
L Mandi
Laboratory of Hydrobiology, Ecotoxicology and Cleansing, Department of Biology, Faculty of Science Semlalia, University Cadi Ayyad, BP 2390, Marrakech, Morocco
M Boko
Interfaculty Centre of Training and Research in Environment for Sustainable Development (CIFRED),
University of Abomey-Calavi, 03 BP 1463, Cotonou, Benin
EE Creppy
Laboratory of Toxicology and Applied Hygiene/UFR of Pharmaceutical Sciences, 146, Road Leo Saignat, 33076 Bordeaux Cedex, France
Published
2016-03-16
Section
Articles

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eISSN: 1684-5315