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African Journal of Biotechnology

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Simple sequence repeat (SSR)-based genetic variability among peanut genotypes different in specific leaf weight and relative water content

Surasak Boontang, Nathpapat Tantisuwichwong, Sanun Jogloy, Chutipong Akkasaeng, Nimitr Vorasoot, Aran Patanothai

Abstract


The objective of this study was to compare if simple sequence repeat (SSR) markers could correctly identify peanut genotypes with difference in specific leaf weight (SLW) and relative water content (RWC). Four peanut genotypes and two water regimes (FC and 1/3 available water; 1/3 AW) were arranged in factorial randomized complete block design with six replications. The data were recorded for specific leaf weight (SLW) and relative water content (RWC), and 256 SSR markers were used to detect genetic difference. 89 SSR markers could detect polymorphism among peanut genotypes (48.9%). The numbers of alleles ranged from 1 to 6 with a mean of 2.7 alleles per locus. The polymorphic information content (PIC) values varied from 0.38 to 0.75 with a mean of 0.48. The genetics relationship among peanut genotypes was estimated. KK 4 was clustered distinct from the others genotypes, whereas ICGV 98324 and ICGV 98303 were grouped in the same cluster furthest from the KK 4. The results from this study could be useful as a source of variation for development of mapping population for drought tolerance in peanut breeding program.

Keywords: Drought, water regime, polymorphism, genetic relationship, SSR markers

African Journal of Biotechnology Vol. 12(26), pp. 4053-4064, 26 June, 2013



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