Controlled transmission of African cassava mosaic virus (ACMV) by Bemisia tabaci from cassava (Manihot esculenta Crantz) to seedlings of physic nut (Jatropha curcas L.)

  • Harry Mensah Amoatey
  • Andrew Sarkodie Appiah
  • Kenneth Ellis Danso
  • Samuel Amiteye
  • Robert Appiah
  • George Yao Percy Klu
  • George Kwabena Owusu

Abstract

Jatropha curcas, a plant with great biodiesel potential is also used to reduce the population of whiteflies, Bemisia tabaci on cassava fields when planted as a hedge. We therefore, investigated the transmission of African cassava mosaic virus (ACMV) by the whitefly vector from cassava to seedlings of 10 accessions of J. curcas as part of a wider investigation on the possible role of J. curcas as an alternative host of ACMV. Transmission tests were conducted in insect-proof cages using adult B. tabaci collected from ACMV-infected cassava in the field, at a rate of three adult whiteflies per J. curcas seedling and a transmission feeding period of four days. Twenty one (21) days after the infestation, leaf samples from individual plants of the 10 J. curcas accessions were tested for the presence of ACMV by the polymerase chain reaction (PCR) and the double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using the monoclonal antibodies SCRI 33. DAS-ELISA detected ACMV in five out of the ten J. curcas accessions while PCR detected it in eight of the 10 accessions. Furthermore, 18 out of the 35 Nicotiana benthamiana indicator plants mechanically inoculated with sap from symptomatic J. curcas seedlings produced symptoms typical of ACMV infection. This indicates that J. curcas is a likely host of ACMV and it may in turn, be able to infect cassava, and presumably other ACMV-susceptible hosts, in the presence of the vector.

Keywords: Jatropha curcas accessions, controlled transmission, mechanical inoculation, enzyme-linked immunosorbent assay, African cassava mosaic virus (ACMV)-susceptible hosts.

African Journal of Biotechnology Vol. 12(28), pp. 4465-4472

Author Biographies

Harry Mensah Amoatey
School of Nuclear and Allied Sciences, University of Ghana, Legon, P. O. Box LG 80, Legon, Accra, Ghana; Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P. O. Box LG 80 Legon, Accra, Ghana.
Andrew Sarkodie Appiah
Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P. O. Box LG 80 Legon, Accra, Ghana
Kenneth Ellis Danso
Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P. O. Box LG 80 Legon, Accra, Ghana
Samuel Amiteye
Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P. O. Box LG 80 Legon, Accra, Ghana
Robert Appiah
Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P. O. Box LG 80 Legon, Accra, Ghana
George Yao Percy Klu
School of Nuclear and Allied Sciences, University of Ghana, Legon, P. O. Box LG 80, Legon, Accra, Ghana.
George Kwabena Owusu
School of Nuclear and Allied Sciences, University of Ghana, Legon, P. O. Box LG 80, Legon, Accra, Ghana.
Published
2016-04-06
Section
Articles

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eISSN: 1684-5315