Biochemical characterization of a thermoactive and thermostable lipase from a newly isolated Trichosporon coremiiforme strain

  • Faouzi Laachari
  • Soumya Elabed
  • Adel Sayari
  • Iraqui Mohammed
  • El Hassan Harchali
  • Abdelhafid Boubendir
  • Saad Koraichi Ibnsouda

Abstract

Nonstop demand for greatly thermostable and thermoactive active lipase encourages the research for the new enzyme sources. In this study, a strain of Trichosporon coremiiforme was isolated from the traditional tannery in the city of Fez in Morocco, lipase production and their lipolytic activity was studied. Pure T. coremiiforme lipase (TCL) was obtained after ammonium sulfate fractionation, G-75 gel filtration and cation exchanger chromatography (Mono-S), having a molecular weight of 67 kDa. TCL presents a maximal activity at pH 8 and 50°C. After a 5 min treatment at 80°C, the enzyme maintained 50% of its activity, which is so far as is known. TCL previously characterized is found to be stable between pH 5 and 10 after 60 min incubation. TCL hydrolyses the long chains triacylglycerols more efficiently than the short ones. A specific activity of 1800 U/mg was measured on tributyrin or olive oil emulsion as substrate. This newly isolated lipase can be considered as a good candidature for industrial and biotechnological applications.

Keywords: Trichosporon coremiiforme, lipase, purification, thermoactive

African Journal of Biotechnology Vol. 12(28), pp. 4503-4511

Author Biographies

Faouzi Laachari
Laboratoire de Biotechnologie Microbienne. Faculté des Sciences et Techniques de Fès- Saïs. Université Sidi
Mohamed Ben Abdellah. B.P. 2202 - Route d’Imouzzer. Fès-Maroc
Soumya Elabed
Laboratoire de Biotechnologie Microbienne. Faculté des Sciences et Techniques de Fès- Saïs. Université Sidi Mohamed Ben Abdellah. B.P. 2202 - Route d’Imouzzer. Fès-Maroc; Université Sidi Mohamed Ben Abdellah, Centre Universitaire Régional d’Interface, Fès- Maroc
Adel Sayari
Laboratoire de Biochimie et de Génie Enzymatique des Lipases. Ecole Nationale d’Ingénieurs de Sfax. BP 3038 Sfax, Tunisie
Iraqui Mohammed
Laboratoire de Biotechnologie Microbienne. Faculté des Sciences et Techniques de Fès- Saïs. Université Sidi Mohamed Ben Abdellah. B.P. 2202 - Route d’Imouzzer. Fès-Maroc.
El Hassan Harchali
Laboratoire de Biotechnologie Microbienne. Faculté des Sciences et Techniques de Fès- Saïs. Université Sidi Mohamed Ben Abdellah. B.P. 2202 - Route d’Imouzzer. Fès-Maroc.
Abdelhafid Boubendir
Département des Sciences de la Nature et de la Vie. Institut des Sciences et Technologie, Centre Universitaire de Mila, Algérie
Saad Koraichi Ibnsouda
Laboratoire de Biotechnologie Microbienne. Faculté des Sciences et Techniques de Fès- Saïs. Université Sidi Mohamed Ben Abdellah. B.P. 2202 - Route d’Imouzzer. Fès-Maroc; Université Sidi Mohamed Ben Abdellah, Centre Universitaire Régional d’Interface, Fès- Maroc
Published
2016-04-06
Section
Articles

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eISSN: 1684-5315