Comparison of protocols for genomic DNA extraction from ‘velame pimenta’ (Croton linearifolius), a native species to the Caatinga, Brazil

  • MM Scaldaferri
  • JS Freitas
  • ESL Santos
  • JGP Vieira
  • ZS Gonçalves
  • CBM Cerqueira-Silva

Abstract

The Caatinga biome occupies some 12% of the Brazilian territory, which is present in at least nine states. The species that constitute its biodiversity have the potential to be used as natural resources, among them are approximately 700 species of the genus Croton. As an example of this potential, the Croton linearifolius specie is used by local communities as a natural insecticide. Associated with the economic potential of the Caatinga species, one must stress the risk of extinction or genetic erosion due to the growing deforestation of natural areas of this biome. These factors make it relevant in genetic studies in order to guide conservation strategies. Considering the lack of molecular studies involving C. linearifolius, we compared the efficiency of six protocols for genomic DNA extraction previously described in literature. The DNA extraction buffers [based on the use of Cetyl trimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), mannitol and sorbitol] were different in their efficiency to obtaining the genomic DNA of C. linearifloius. In general, protocols using CTAB buffer were more efficient. The use of liquid nitrogen in the maceration process was also evaluated and its use was considered a no necessary factor in obtaining DNA in adequate quantity and quality for PCR platform procedures.

Keywords: DNA Isolation, molecular markers, native species of Caatinga

African Journal of Biotechnology Vol. 12(30), pp. 4761-4766

Author Biographies

MM Scaldaferri
Laboratory of Applied Molecular Genetics, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil
JS Freitas
Laboratory of Applied Molecular Genetics, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil
ESL Santos
Laboratory of Applied Molecular Genetics, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil; Laboratory of general biology and botany, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil
JGP Vieira
Laboratory of Applied Molecular Genetics, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil
ZS Gonçalves
Laboratory of Applied Molecular Genetics, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil
CBM Cerqueira-Silva
Laboratory of Applied Molecular Genetics, Departamento de Estudos básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga - Ba, 45700-000, Brazil
Published
2016-04-12
Section
Articles

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eISSN: 1684-5315