Chemical composition and antioxidant activity of essential oil, various organic extracts of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco

  • H Zidane
  • M Elmiz
  • F Aouinti
  • A Tahani
  • J Wathelet
  • M Sindic
  • A Elbachiri

Abstract

In the present work, we studied the chemical composition of the essential oil of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco. The essential oils were obtained by hydrodistillation and their chemical composition was analysed using gas chromatography- mass spectrometry (GC–MS). Camphene, borneol, cyclohexanol-2, 2, 6 tremethyl, terpineol-4 and α-pinene were the main constituents of the essential oil of C. ladanifer, while in the essential oil of C. libanotis we obtained terpineol-4, γ-terpinene, camphene, sabinene, α-terpinene and α-pinene. The antioxidant potential of various extracts (water, ethanol, ethanol: water (50:50), methanol, methanol: water (50:50), acetonitrile) and essential oils of C. ladanifer and C. libanotis were carried out by the method of 1,1-diphenyl-1-picrylhydrazylhydrate (DPPH) free radical scavenging. Total phenolic and flavonoid contents were determined. The result show that C. ladanifer of the leaves of methanol: water (50:50) extract had the highest value of total phenolic content and the lowest was present in ethanol: water (50:50) extract of the stem and acetonitrile extract of the flowers of C. libanotis. From our experimental results, the extract of flowers, fruit, stem and leaves of those plants showed highest potential as free radical scavengers.

Keywords: Antioxidant, phenolics, flavonoids, essential oil, extracts, gas chromatography- mass spectrometry (GC–MS).

African Journal of Biotechnology Vol. 12(34), pp. 5314-5320

Author Biographies

H Zidane
Laboratory of Physical Chemistry of the Natural Resources and Environment, University Mohammed Premier, BP 717, 60000 Oujda, Morocco
M Elmiz
Laboratory of Physical Chemistry of the Natural Resources and Environment, University Mohammed Premier, BP 717, 60000 Oujda, Morocco
F Aouinti
Laboratory of Physical Chemistry of the Natural Resources and Environment, University Mohammed Premier, BP 717, 60000 Oujda, Morocco
A Tahani
Laboratory of Physical Chemistry of the Natural Resources and Environment, University Mohammed Premier, BP 717, 60000 Oujda, Morocco
J Wathelet
Unity of General and Organic Chemistry, Faculty of the Agronomic Sciences of Gembloux, Passage of the Prisoners 2, B-5030 Gembloux, Belgium
M Sindic
Unity of General and Organic Chemistry, Faculty of the Agronomic Sciences of Gembloux, Passage of the Prisoners 2, B-5030 Gembloux, Belgium
A Elbachiri
Laboratory of Physical Chemistry of the Natural Resources and Environment, University Mohammed Premier, BP 717, 60000 Oujda, Morocco
Published
2016-05-17
Section
Articles

Journal Identifiers


eISSN: 1684-5315