Expression of an engineered tandem-repeat starch-binding domain in sweet potato plants

  • Yunfeng Zhang
  • Qin Ji
  • Yujun Xing
  • Qiang Li
  • Xin Wang
  • Chun Xu
  • Cuixiang Huang

Abstract

In this study, the transgenic sweet potato Xu55-2 modified with an engineered tandem repeat of a family 20 starch binding domain (SBD2) was analyzed by Western dot blot to investigate whether SBD2 proteins are capable of granule-targeting during starch biosynthesis. Furthermore, the impact of SBD2 accumulation in granules on the physicochemical properties of the transgenic starches was also investigated. Our results demonstrate that the high levels of SBD2 protein could be accumulated in granules. The SBD2 expression affect granule morphology without altering the primary structure of the constituent starch molecules, suggesting that SBD2 could be used as an anchor for effector proteins to sweet potato starch granules during biosynthesis.

Keywords: Sweet potato (Ipomoea batatas), tandem starch-binding domain, transgenic starch, granule morphology.

African Journal of Biotechnology Vol. 12(41), pp. 5994-5999

Author Biographies

Yunfeng Zhang
The School of Life Sciences, Huaiyin Normal University, Huaian 223300, China
Qin Ji
The School of Life Sciences, Huaiyin Normal University, Huaian 223300, China
Yujun Xing
The School of Life Sciences, Huaiyin Normal University, Huaian 223300, China; College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Qiang Li
Sweet potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221121, China.
Xin Wang
Sweet potato Research Institute, Chinese Academy of Agricultural Science, Xuzhou 221121, China.
Chun Xu
The School of Life Sciences, Huaiyin Normal University, Huaian 223300, China.
Cuixiang Huang
The School of Life Sciences, Huaiyin Normal University, Huaian 223300, China.
Published
2016-06-06
Section
Articles

Journal Identifiers


eISSN: 1684-5315