Optimization of culture variables for the production of L- asparaginase from Pectobacterium carotovorum

  • Barkha Singhal
  • Kamendra Swaroop


L-Asparaginase is an anti-neoplastic agent used in the chemotherapy of acute lymphoblastic leukemia. The present work deals with production of intra-cellular L-asparaginase from Pectobacterium carotovorum using submerged fermentation. The statistical approaches of Plackett-Burman and response surface methodology were applied to optimize the culture parameters for the production of L-asparaginase through a bacterium P. carotovorum MTCC 1428. Optimal conditions for maximal productivity were determined based on six parameters at two different levels. Initially, a screening design methodology was used to evaluate the process variables, and the response surfaces applied to find optimal concentration for production. The analysis revealed that the optimum levels of L-asparagine, lactose and inoculum percentage were found to be 4.00, 3.5 g L−1, and 2.75%, respectively. The maximum enzyme activity in the optimized medium was 19.33 U ml−1.

Keywords: Design of experiments (DoE), L-asparaginase, response surface methodology, Plackett-Burman design and acute lymphoblastic leukemi.

Afr. J. Biotechnol Vol. 12 No. 50

Author Biographies

Barkha Singhal
School of Biotechnology, Gautam Buddha University, Greater Noida, India
Kamendra Swaroop
School of Biotechnology, Gautam Buddha University, Greater Noida, India

Journal Identifiers

eISSN: 1684-5315