Comparison of protease production from newly isolated bacterial strains T₅, T₃ and H₃ after immobilization

Abstract

Protease has gained a very important position in many industries such as food, pharmaceutical, chemical and leather industries. In this research, protease was obtained from bacteria. The bacterial strain was obtained from soil which was collected from different areas of Lahore, Pakistan. Fermentation medium (by using sub-merged fermentation technique) was incubated for 48 h at 37°C temperature and agitation speed of 200 rpm. The protease was partially purified with 70% ammonium sulphate. Four different supports were used for the immobilization of the bacterial protease by physical adsorption method. When partially purified protease was immobilized on Amberlite (XAD 761), its production amount was 371.42% (52 U/ml/g support) with strain T₅; 208.33% (25 U/ml/g support) with strain T₃ and 342.24% (64 U/ml/g support) with strain H₃; when it was immobilized on Duolite (A568), its amount of production was 314.28% (44 U/ml/g support) with strain T₅; 225% (27 U/ml/g support) with strain T₃ and 395.72% (74 U/ml/g support) with strain H₃; when it was immobilized on Lewatit (VPOC 1600), the amount of production was 450% (63 U/ml/g support) with strain T₅; 541.66% (65 U/ml/g support) with strain T₃ and 320.85% (60 U/ml/g support) with strain H₃. But, when it was immobilized on Pentynyl Dextran (NT4L360), its amount of production was 271.42% (38 U/ml/g support) with strain T₅, 3483.33% (418 U/ml/g support) with strain T₃ and 304.81% (57 U/ml/g support) with strain H₃. Immobilized protease from bacterial strain T₃ had the highest immobilizing activity of 3483.33% (418 U/ml/g support). This immobilized protease with the highest activity could be used in food, pharmaceutical and leather industries.

Keywords: Protease, bacterial strain, immobilizing activity