African Journal of Biotechnology

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Production of extra-cellular polymer in Azotobacter and biosorption of metal by exopolymer

Giti Emtiazia, Zahra Ethemadifara, Mohammad H Habibib


Two Azotobacter strains were isolated from alkaline and acid soils. The production of alginate and exopolymer from these two strains showed that, strain AC2 produced high polymer in 2% beet molasses or 1% sucrose broth and addition of nitrogen sources (yeast extract) reduced production of this polymer. The optimum condition for production of maximum polymer production (7.5 mg/ml) was at 200 rpm shaking, pH 7 without addition of nitrogen sources. The production of polymer was reduced at pH 4. The polymer adsorbed Cu, Zn, and Fe, at 15.5, 20 and 25 mg, respectively.
Key Words: Azotobacter, exopolymer, of extracellular polysaccharide, biosorption of metal.
African Journal of Biotechnology Vol.3(6) 2004: 330-333
AJOL African Journals Online