Saccharification of banana agro-waste by cellulolytic enzymes

  • MMV Baig Botany Research Laboratory & Plant Disease Clinic, Science College, Nanded 431605 India.
  • MLB Baig Botany Research Laboratory & Plant Disease Clinic, Science College, Nanded 431605 India.
  • MIA Baig Department of Agronomy, College of Agriculture, Marathwada Agricultural University, Parbhani 431402, India
  • Majeda Yasmeen Botany Research Laboratory & Plant Disease Clinic, Science College, Nanded 431605 India
Keywords: Banana agrowaste, Trichoderma lignorum, cellulases, saccharification

Abstract

Banana is major cash crop of this region generating vast agricultural waste after harvest. The agro-waste including dried leaves and psuedostem after harvest was used as substrate for the release of sugars. Saccharification of banana agro waste by cellulases of Trichoderma lignorum was investigated. The steam treated agro-waste yielded 1.34 mg/ml of reducing sugars after 24 hr. The size of substrate affected saccharification where the smaller size (<120 µ) yield more sugars. Maximum sugars were released at pH 6.0 whereas 40°C was the optimum temperature. Thus, under these conditions the agro waste left behind for natural degradation can be utilized affectively to yield fermentable sugars which can be converted into other substances like alcohol.
Key Words: Banana agrowaste, Trichoderma lignorum, cellulases, saccharification.
African Journal of Biotechnology Vol.3(9) 2004: 447-450
Published
2005-03-21
Section
Articles

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eISSN: 1684-5315