Effect of cultivation conditions on growth and antifungal activity of Mycena leptocephala

  • H Vahidi School of Pharmacy, Shaheed Beheshti University of Medical Science, 10 Shams alley, Across from Day Hospital, Vali-e- Asr street, P.O. Box 14155-6153 Tehran, Iran.
  • F Kobarfard Department of Medicinal chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences P.O. Box 14155-6153 Tehran, Iran.
  • F Namjoyan School of Pharmacy, Shaheed Beheshti University of Medical Science, 10 Shams alley, Across from Day Hospital, Vali-e- Asr street, P.O. Box 14155-6153 Tehran, Iran.
Keywords: Culture conditions, basidiomycetes, bioactive compounds, carbon source, nitrogen source and temperature

Abstract

Growth and production of antifungal agent by Mycena leptocephala was investigated in different culture media composition at various initial pH and temperatures. Maximum growth and activity was observed at the initial pH of 5.5 and 25oC. No detectable growth and activity was observed at pH of 3.5 and 7.5. Growth of the fungus and antifungal activity were also very low at 37oC and 20oC. The organism grew better and culture extract showed higher level of antifungal activity when malt extract plus glucose were used as carbon source and yeast extract was used as nitrogen source. Meanwhile the lowest level of growth and antifungal activity were observed when starch was used as carbon source for growth of the fungus.
Key Words: Culture conditions, basidiomycetes, bioactive compounds, carbon source, nitrogen source and temperature.
African Journal of Biotechnology Vol.3(11) 2004: 606-609
Published
2005-03-23
Section
Articles

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eISSN: 1684-5315