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African Journal of Biotechnology

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Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera, Ephestia kuehniella Zeller

Maher Obeidat, Dhia Hassawi, Ihab Ghabeish

Abstract


Bacillus thuringiensis was investigated in four different habitats (grain dust, olive-cultivated soils, waste and industrial-byproducts contaminated soils, and animal byproducts-contaminated soils). The bacterium was highly abundant in soils contaminated with animal byproducts. Eight serotypes with Bacillus thuringiensis israelensis being the most common. Out of the twenty-six isolated strains, five strains (serotype: kenyae, kurstaki, kurstaki HD1 and thuringiensis) that produced bipyramid crystal proteins were toxic to the lepidoptera larvae of Ephestia kuehniella Zeller. The SDS-PAGE protein profile analysis showed a relationship between the crystal protein shape and the toxicity to the larvae of the tested insect.
Key Words: Bacillus thuringiensis serotypes, Ephestia kuehniella, parasporal crystal proteins.
African Journal of Biotechnology Vol.3(11) 2004: 622-626



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