Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera, Ephestia kuehniella Zeller

  • Maher Obeidat Al-Balqa’ Applied University, Faculty of Agricultural Technology, Biotechnology Department, Al-Salt 19117, Jordan
  • Dhia Hassawi Al-Balqa’ Applied University, Faculty of Agricultural Technology, Biotechnology Department, Al-Salt 19117, Jordan
  • Ihab Ghabeish Al-Balqa’ Applied University, Faculty of Al Salt, Al-Salt 19117, Jordan.
Keywords: Bacillus thuringiensis serotypes, Ephestia kuehniella, parasporal crystal proteins

Abstract

Bacillus thuringiensis was investigated in four different habitats (grain dust, olive-cultivated soils, waste and industrial-byproducts contaminated soils, and animal byproducts-contaminated soils). The bacterium was highly abundant in soils contaminated with animal byproducts. Eight serotypes with Bacillus thuringiensis israelensis being the most common. Out of the twenty-six isolated strains, five strains (serotype: kenyae, kurstaki, kurstaki HD1 and thuringiensis) that produced bipyramid crystal proteins were toxic to the lepidoptera larvae of Ephestia kuehniella Zeller. The SDS-PAGE protein profile analysis showed a relationship between the crystal protein shape and the toxicity to the larvae of the tested insect.
Key Words: Bacillus thuringiensis serotypes, Ephestia kuehniella, parasporal crystal proteins.
African Journal of Biotechnology Vol.3(11) 2004: 622-626
Published
2005-03-23
Section
Articles

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eISSN: 1684-5315