Extracts of Pterocarpus osun as a histological stain for collagen fibres

  • O G Avwioro Department of Histopathology, School of Medical Lab. Science, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria
  • P C Aloamaka Department of Physiology, Ambrose Alli University, Ekpoma, Nigeria
  • N U Ojianya Department of Histopathology, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria
  • T Oduola Department of Haematology and Blood Transfussion, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria
  • E O Ekpo Department of Anatomy, Ladoke Akintola University, Ogbomosho, Nigeria
Keywords: Pterocarpus osun, phytochemical screening, tissue sections, stain, mordant

Abstract

The staining ability of Pterocarpus osun extract on tissue sections was determined. 2 kg of P. osun stem was dried, milled to obtain a fine powder and a red pigment extracted from the powder with 1 L of 70% ethanol at 78°C for 24 h. The alcoholic and acidic extracts were used to stain tissue sections. Collagen fibres, red blood cells and muscles were stained in shades of reddish brown. Preliminary phytochemical screening of this extract revealed that it contained alkaloids, steroid ring, cardiac glycosides and reducing sugar.

African Journal of Biotechnology Vol. 4 (5), pp. 460-462, 2005
Published
2005-08-16
Section
Articles

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eISSN: 1684-5315