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Published:
Aug 16, 2005Keywords:
Pterocarpus osun phytochemical screening tissue sections stain mordant
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Avwioro, O. G., Aloamaka, P. C., Ojianya, N. U., Oduola, T., & Ekpo, E. O. (2005). Extracts of Pterocarpus osun as a histological stain for collagen fibres. African Journal of Biotechnology, 4(5), 460–462. https://doi.org/10.4314/ajb.v4i5.15122
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Extracts of Pterocarpus osun as a histological stain for collagen fibres
Abstract
The staining ability of Pterocarpus osun extract on tissue sections was determined. 2 kg of P. osun stem was dried, milled to obtain a fine powder and a red pigment extracted from the powder with 1 L of 70% ethanol at 78°C for 24 h. The alcoholic and acidic extracts were used to stain tissue sections. Collagen fibres, red blood cells and muscles were stained in shades of reddish brown. Preliminary phytochemical screening of this extract revealed that it contained alkaloids, steroid ring, cardiac glycosides and reducing sugar.
African Journal of Biotechnology Vol. 4 (5), pp. 460-462, 2005
African Journal of Biotechnology Vol. 4 (5), pp. 460-462, 2005
