In vitro propagation of Stevia rebaudiana Bert in Bangladesh

  • MS Uddin
  • MSH Chowdhury
  • MMMH Khan
  • MB Uddin
  • R Ahmed
  • MdA Baten
Keywords: In vitro propagation, 2, 4-D, Stevia rebaudiana

Abstract

An experiment was conducted on in vitro culture of Stevia rebaudiana Bert, an important non-caloric sweetening herb to explore its potential for micro-propagation. Leaf, nodal and inter-nodal segments of the selected herb as explant were cultured on MS medium containing 2,4-D at 2, 3, 4 and 5 mg/L for callus induction. Inter-nodal segments initiated callus earlier than node and leaf. The highest amount of callus was found in MS medium with 3.0 mg/L 2,4-D and MS medium with 5.0 mg/L 2,4-D gave the poorest callus
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eISSN: 1684-5315