In vitro flowering in embryogenic cultures of Kinnow mandarin (Citrus nobilis Lour ´ C. deliciosa Tenora)
Keywords: Embryogenic callus, in vitro flowering, Kinnow (Citrus nobilis ´ C. deliciosa), ovule culture
AbstractEmbryogenic cultures of Kinnow mandarin (C. nobilis Lour × C. deliciosa Tenora) were raised from unfertilized ovules dissected from unopened flower buds of this plant inoculated on MS medium supplemented with 2 mg/L kinetin (KN). In vitro flowering was induced in these cultures by using
different concentrations of KN and sucrose as well as subjecting these cultures to different photoperiods. Maximum percentage (31.94%) of cultures producing flowers and maximum number (5.58) of flowers per culture was observed on MS medium supplemented with KN (2 mg/L) and sucrose
40 g/L at 12-h photoperiod.
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