African Journal of Biotechnology

The AJOL site is currently undergoing a major upgrade, and there will temporarily be some restrictions to the available functionality.
-- Users will not be able to register or log in during this period.
-- Full text (PDF) downloads of Open Access journal articles will be available as always.
-- Full text (PDF) downloads of subscription based journal articles will NOT be available
We apologise for any inconvenience caused. Please check back soon, as we will revert to usual policy as soon as possible.

Comparative genome analysis of trypanotolerance QTL

J Nganga, M Imbuga, FA Iraqi


Certain breeds of domestic ruminants show remarkable resistance to the effects of African trypanosomosis. Unlike susceptible animals, trypanotolerant animals control parasitemia and do not show
severe anaemia or production loss. Identification of trypanotolerance genes in cattle is hampered by cost and breeding time. Marked differences between inbred strains of mice in their response to T.
congolense infection can be exploited in the analysis of the genetic basis of the infection. Murine trypanotolerance QTLs have been identified on chromosome 17, 5 and 1, and designated as Tir1, 2 and
3, respectively. Tir1 and 2 have been fine mapped to a confidence interval of 1 cM. In order to find the mouse homologous region on the bovine genome, nucleotide sequence across 95% CI of Tir2 and 3
were used in the selection of candidate genes. Homologous sequences were used in the definition of synteny relationships and subsequent identification of the shared disease response genes. The homologous genes within the human genome were then identified and aligned to the bovine radiation hybrid map in order to identify the mouse/bovine homologous regions. This revealed homology between murine and bovine QTL on Tir3 while the region on Tir2 is linked to innate immune response.

AJOL African Journals Online