Purification, characterization and application of laccase from Trametes versicolor for colour and phenolic removal of olive mill wastewater in the presence of 1- hydroxybenzotriazole

  • RC Minussi
  • MA Miranda
  • JA Silva
  • CV Ferreira
  • H Aoyama
  • S Marangoni
  • D Rotilio
  • GM Pastore
  • N Durán
Keywords: Laccase, Trametes versicolor, 1-hydroxybenzotriazole, mediator, olive mill wastewater.


Laccase forms (L1 and L2) from Trametes versicolor CCT 4521 showed a molecular mass of 66 kDa and optimum temperature around 40oC. The optimum pH (4.0 and 5.0) and Km (28.6 and 5 ìM) values using
syringaldazine as substrate were found for L1 and L2, respectively. The enzymes were able to oxidize several compounds and were strongly inhibited by sodium azide, L-cysteine and dithiothreitol. The 75%
of the N-terminal sequences were identical in both forms and similarities around 40 - 60% of laccases from wood-degrading fungi were observed. The use of 1-hydroxybenzotriazole as a mediator increased the compounds oxidized by laccases in olive mill wastewater.

Journal Identifiers

eISSN: 1684-5315