African Journal of Biotechnology

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Development of a high-throughput microsatellite typing approach for forensic and population genetic analysis of wild and domestic African Bovini.

BJ Greyling, P Kryger, S du Plessis, WF van Hooft, P van Helden, WM Getz, ADS Bastos


Conservation management and forensic traceability of African buffalo and cattle rely on the timely provision of unbiased and accurate genetic information. An approach in which 17 cattle microsatellite
markers are co-electrophoresed, following amplification in three core multiplex reactions was established for this purpose. Mean allelic richness per locus was 8.24 and 6.47, for buffalo and Bonsmara cattle, respectively, whilst an unbiased match probability of 6.5x10-17 and 1.03 x 10-16 was
obtained for each. These results confirm the usefulness of this rapid, cost-effective typing approach for forensic, paternity and fine-scale genetic analyses of wild and domestic African Bovini tribe members.

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