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Induction of callus and extraction of alkaloid from Yi Mu Cao (<i>Leonurus heterophylus Sw.</i>) culture
Abstract
High efficient callus was induced from cotyledon and hypocotyls of Leonurus heterophyllus Sw. on Murashige and Skoog (MS) medium containing 2 mg/l 6-benzylaminopurine (6-BA) and 0.5 mg/l 2,4-
dichlorophenxyacetic acid (2,4-D). The induction rate reached up to 94.2% in cotyledon explants and 99.2% in hypocotyls. They formed yellowish loose callus when they were subcultured on MS medium
with 2 mg/l 6-BA and 0.5 mg/l naphtaleneacetic acid (NAA). Suspension culture system was established as the callus transferred to MS liquid medium supplemented with 2 mg/l 6-BA and 0.5 mg/l NAA, and the
maximum biomass is obtained on the 16th day. The content of alkaloid extracted from suspension culture callus was only 0.081%, but the addition of 0.03 mg/l L-proline (L-Pro) could increase the content up to 2.1%.
dichlorophenxyacetic acid (2,4-D). The induction rate reached up to 94.2% in cotyledon explants and 99.2% in hypocotyls. They formed yellowish loose callus when they were subcultured on MS medium
with 2 mg/l 6-BA and 0.5 mg/l naphtaleneacetic acid (NAA). Suspension culture system was established as the callus transferred to MS liquid medium supplemented with 2 mg/l 6-BA and 0.5 mg/l NAA, and the
maximum biomass is obtained on the 16th day. The content of alkaloid extracted from suspension culture callus was only 0.081%, but the addition of 0.03 mg/l L-proline (L-Pro) could increase the content up to 2.1%.
