Isolation, identification and screening of potential xylanolytic enzyme from litter degrading fungi

  • M Palaniswamy
  • BV Pradeep
  • R Sathya
  • J Angayarkanni
Keywords: Litter degrading fungi, xylanolytic enzyme, xylanase, b-xylosidase.

Abstract

Consortia of litter degrading fungal species were developed from different baiting substrates collected in and around Western ghat forest ecosystem, Coimbatore, Tamilnadu, India. Fifty-three litter degrading
fungal species were isolated by nylon litterbag technique. The production of endo-b-1,4-xylanase (1,4-b-D-xylan xylanohydrolase, E.C. 3.2.1.8), b-D-xylosidase (1,4-b-xylan xylanohydrolase, E.C. 3.2.1.37) and protease was studied using oat spelt xylan as carbon source. Results showed that all fifty-three fungal species isolated from various litter samples produced fairly good xylanolytic enzyme activity. The xylanase and b-D-xylosidase activity ranges from 4.41 to 132.20 U and 48.72 to 1510.32 U, respectively. Growth was determined in terms of mycelial dry weight, which ranged between 0.209 and 1.047 mg/ml. The protease enzyme activity was from 19.7 to 60.8 U. This is the first report concerning xylanolytic
enzyme production by the litter degrading fungi, isolated from litter samples.
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