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Biodegradation of 2,4-dichlorophenol using <i>Mycoplana dimorpha</i> extracts and evaluation of kinetic parameters


R Manikandan
HJ Prabhu
P Sivashanmugam

Abstract

Twenty seven combinations of process variables were developed and used to produce crude extracts of Mycoplana dimorpha. Crude extracts containing 2,4-dichlorophenol degrading enzymes, were immobilized on sodium alginate beads and degradation studies was conducted in a packed bed column. The rate of degradation of 2,4-dichlorophenol by immobilized crude extracts of was measured at different time intervals and it was found that 82 to 86% of 2,4-dichlorophenol can be decomposed with different initial concentrations in 30 min. The Km and Vmax values were determined.

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eISSN: 1684-5315