Biodegradation of 2,4-dichlorophenol using Mycoplana dimorpha extracts and evaluation of kinetic parameters

  • R Manikandan
  • HJ Prabhu
  • P Sivashanmugam
Keywords: 2, 4-Dichlorophenol, degradation, crude extract, Mycoplana dimorpha, immobilization, packed bed column.


Twenty seven combinations of process variables were developed and used to produce crude extracts of Mycoplana dimorpha. Crude extracts containing 2,4-dichlorophenol degrading enzymes, were immobilized on sodium alginate beads and degradation studies was conducted in a packed bed column. The rate of degradation of 2,4-dichlorophenol by immobilized crude extracts of was measured at different time intervals and it was found that 82 to 86% of 2,4-dichlorophenol can be decomposed with different initial concentrations in 30 min. The Km and Vmax values were determined.

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eISSN: 1684-5315