DNA damage by the cobalt (II) and zinc (II) complexes of tetraazamacrocyclic in Tetrahymena thermophila

  • Z Xu
  • R Shi
  • W Wang
  • R Tao
  • J Bi
  • Z Wei
Keywords: Tetraazamacrocycle Zn(II) complex, tetraazamacrocycle Co(II) complex, Tetrahymena thermophila, DNA damage, the comet assay.

Abstract

Using the single cell gel electrophoresis method, the tetraazamacrocycle Zn(II) complex (Zn(II)-L) and the tetraazamacrocycle Co(II) complex (Co(II)-L) were investigated focusing on their DNA damage to Tetrahymena thermophila. When the cells were treated with the 0.05, 0.25 and 0.50 mg/ml Zn(II)-L, the tail length increased significantly, with 10.83, 11.56 and 11.87 m, respectively. With the dose of 0.5
mg/ml Zn(II)-L treatment, 45.5% cells distributed in grade 3. After treatment with the 0.05, 0.25 and 0.50 mg/ml Co(II)-L, the tail length of the cells also increased significantly, with the length of 15.64, 17.75 and
19.21 m, respectively. When treated with 0.5 mg/ml Co(II)-L, 98.1% cells showed tail and 75.6% cells distributed in grade 3. The results indicated that Co(II)-L induced a relatively high level of DNA damage
in comparison with the level of damage induced by Zn(II)-L.
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eISSN: 1684-5315