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Plant regeneration through organogenesis and shoot proliferation in <i>Trichodesma indicum</i> (Linn) R. Br. – a medicinal herb


N Verma
V Koche
KL Tiwari
SK Mishra

Abstract

Adhahpuspi (Trichodesma indicum, Family – Boraginaceae) is a cross-pollinated species. Its complete regeneration was accomplished through in vitro techniques. The zygotic embryos placed on MS (Murashige and Skoog) medium fortified either with kinetin, BA (N6-benzyl Aminopurine) or NAA (∝-naphthalene acetic acid) produced callus and adventitious shoots; whereas those placed on MS medium supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid) formed callus. On subculture, the
nodal pieces produced axillary shoots that were suitable for further propagule proliferation. Rhizogenesis occurred in 60% micro shoots treated with IBA (indole-3-butyric acid) pulse. The regenerated plants successfully acclimatized and started flowering in green house maintained at 30 ± 2ºC temperature and 70% RH.

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eISSN: 1684-5315