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Regeneration and molecular analysis of date palm (<i>Phoenix dactylifera</i> L.) plantlets using RAPD markers

O Ahmed
B Chokri
D Noureddine
M Mohamed
T Mokhtar


Clonal plants of date palm (Poenix dactylifera L.) were regenerated from juvenile leaves on regimes consisting of the use of 2,4-D. Success depended on the concentrations of 2,4-D tested. The cultures
produced adventitious shoot buds directly at the basal part of leaves as well as excessive calli. Somaclonal variation in plantlets which can be induced by 2,4-D during recurrent somatic embryogenesis were tested by random amplified polymorphic DNA (RAPD) profiles. Nine arbitrary 10-
mer primers were used to amplify DNA from 180 plantlets. RAPD patterns of the plantlets were identical with the original plant mother, indicating that 2,4-D did not induce somaclonal variation that can be detected by the RAPD technique.