Complete nucleotide sequencing, SNP identification and characterization of SRY gene in Indian Sangamneri goat

  • K Prashan
  • DS Gour
  • PP Dubey
  • A Jain
  • DK Nanda
  • BK Joshi
  • D Kumar
Keywords: Goat, HMG box, SSCP, SRY gene.

Abstract

The SRY gene is responsible for testis determination in mammals. It codes for a protein with a DNA binding domain similar to those found in high-mobility group protein (HMG box). Mutation in this gene leads to Gonadal Dysgenesis an abnormal sexual phenotype described in human, cattle, horse, and river buffalo. Here we report PCR-SSCP analysis and sequencing of coding region of SRY gene in Sangamneri goat (Capra hircus) using 40 male goats (bucks) samples. Polymorphism was detected in
only one sample indicating that a major portion of SRY gene is highly conserved in this breed.
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eISSN: 1684-5315