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African Journal of Biotechnology

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RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India

R.K Garg, N Silawat, P Sairkar, N Vijay, N.N Mehrotra

Abstract


The random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation of the 2 populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. 10 random 10-mer primers were primarily scored in 3 individuals from each of the 2 locations. Five primers, which gave polymorphism, were selected for polymerase chain reaction (PCR) and used in the final RAPD analysis of 20 individuals collected from 2 sites, namely Bhadwada reservoir (Bhopal) and Mohinisagar reservoir (Gwalior). These primers produced 388 scorable DNA fragments were found, of which 252 (64.98%) were polymorphic, 182 (46.90%) were monomorphic and 14 (3.61%) were unique. RAPD banding patterns, showed variations between and within the populations, while, the morphological variations were negligible.



AJOL African Journals Online