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African Journal of Biotechnology

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Antimicrobial activity of photo-activated cow urine against certain pathogenic bacterial strains

RK Upadhyay, P Dwivedi, S Ahmad

Abstract


In the present investigation binary combination of photo activated and it binary combinations was determined against seven bacterial strains. Photoactivated cow urine has shown MIC value 0.25 ml/ml MIC value against Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 11778), Lactobacillus acidophilus (ATCC 53103 and Micrococcus luteus (ATCC 9341), while it was found 0.125 ml/ml against E. coli (ATCC 25922). Binnary combinations of cow urine with Neem and Bavchi oil has shown synergistic effect as the MIC value obtained was 0.125-0.25 ml/ml. Similarly photoactivated cow urine has shown least MBC value that is, 0.25-1.0 ml/ml. Further, growth inhibition zone diameters obtained in presence of photoactivated cow urine and its binary combinations were found much larger than antibiotic drugs. Neem oil has shown highest inhibition zone diameter that is, 45 mm against S. pneumoniae. Neem oil
and cow urine have shown 33 to 35 mm inhibition zone diameter against B. cereus, L. acidophilus, Micrococcus. Luteus, K. pneumoniae and S. pneumoniae, while Bawchi oil and cow urine combination has shown high diameter inhibition zone diameter that is 41 mm against K. pnumoniae. Photoactivated cow urine has shown 32 to 36 mm inhibition zone diameter homogeneously against all bacterial strains. It proves very high antimicrobial susceptibility of cow urine and essential oils.



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