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greater protease production (2927 U/ml) in medium containing only whole Sardinelle powder than that obtained in control medium (2800 U/ml). Proteases were produced even when strain NH1 was cultivated in medium containing only powder prepared from combined heads and viscera Sardinelle (CHVSP), about 2117 U/ml. Protease production was also carried out in media containing hulled grain of wheat, a by-product of semolina factories, as carbon source. Maximum activity (2517 U/ml) was achieved when the strain was grown in medium containing hulled grain of wheat (10 g/l), casein peptone (2 g/l), K2HPO4 (0.5 g/l) and KH2PO4 (0.5 g/l). Moreover, protease production was considerably enhanced when the
strain was grown in medium containing both hulled grain of wheat and CHVSP as carbon and nitrogen sources, respectively, (4771 U/ml). The study shows that hulled grain of wheat and powders from fishery by-products could be utilized as bacterial substrates for the production of alkaline proteases by B. licheniformis NH1.