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blight. Infected tissues were washed with sterile distilled water and crushed in peptone water. Then 50 ìl of the extract were cultured on King’s B and NA media containing cyclohexamide (50 ìg/ml). After 48 to 72 h, bacterial colonies were selected and purified. On the basis of morphological, physiological and biochemical characteristics, pathogenicity and PCR with specific primers, the isolated were placed in two groups. The first group consists of 20 isolates that caused leaf blight, identified as P. syringae pv.
Syringae, while the second group is made up of 18 isolates that caused basal glumes blotch identified as P. syringae pv. atrofaciens. This is the first report of the existence of P. syringae pv. atrofaciens on wheat in Iran.