Anti-malarial activity of ethanolic leaf extract of <i>Piliostigma thonningii</i> Schum. (Caesalpiniacea) in mice infected with <i>Plasmodium berghei berghei</i>

Abstract

Piliostigma thonningii Schum. (Caesalpiniacea) is used traditionally in the management of fever, cough, wounds and various ulceration. Oral acute toxicity of the ethanolic leaf extract of Piliostigma thorningii was evaluated in mice using modified Lorke’s method. The ethanolic leaf extract was evaluated for in vivo anti plasmodial activity against chloroquine sensitive strain of Plasmodium berghei berghei NK65 in mice. Four day suppressive, curative effect against established infection and prophylactic models of anti plasmodial studies were carried out. The oral median lethal dose was determined to be 3807.89 mg/kg body weight. The extract (100,200 and 400 mg/kg) exerted dose dependent chemo suppressive effects at the different levels of the infections tested. However the anti-plasmodial effect of chloroquine at 5 mg/kg body weight was higher than the extract in all the test models. This