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African Journal of Biotechnology

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Construction of a high-EGFR expression cell line and its biological properties comparing with A431 cell

Y Zhang, X Li, X Xu, W-J Luo

Abstract


Targeted screening of EGFR compounds has become one of the medical research focuses for tumor therapy. A431, which naturally expresses high levels of EGFR, was compared with the stably high expressing EGFR cell line HEK293. Flow cytometry was used to analyze cell growth and Western blot
was used to determine expression of EGFR, p-EGFR and p-ERK. Cellular membrane chromatography analysis revealed longer on-peak chromatographic and greater on-peak area of A431. Results demonstrated that the high-expression EGFR cell line A431 improved sensitivity of cellular membrane chromatography when screening drugs targeted to EGFR.



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