Development of a new medium containing date syrup for production of bleomycin by Streptomyces mobaraensis ATCC 15003 using response surface methodology

  • Hesham H Radwan
  • Fars K Alanazi
  • Ehab I Taha
  • Hamdy A Dardir
  • Ihab M Moussa
  • Ibrahim A Alsarra

Abstract

A combined statistical approach of orthogonal design and polynomial regression were applied to optimize the composition and concentration of a liquid fermentation medium for the production of bleomycin (BLM) by Streptomyces mobaraensis. Optimal conditions for maximal productivity were determined based on eight parameters at three different levels. The sources of carbon and nitrogen concentration and their interactions with other precursors were found to be statistically significant factors. When date syrup was used as an additional carbon source, higher BLM amount was obtained in comparison to glucose. It was found that the optimum nitrogen source was achieved with the use of soyabean meal. The combined orthogonal design and response surface methodology predicted optimal conditions for production of BLM to be 138 mg dl-1. A confirmatory experiment of the optimal medium composition produced 142 mg dl-1 in the fifth day fermentation at 30°C. The complex medium containing 40 gml-1 date syrup as additional carbon source enhanced the production of BLM by 73%. The combined statistical approach enabled rapid identification and integration of key medium parameters for optimizing secondary metabolite production and could be very useful in pharma-ceutical screening programs.

Keywords: Bleomycin, Streptomyces mobaraensis, orthogonal design, medium optimization, date syrup

African Journal of Biotechnology Vol. 9(33), pp. 5450-5459, 16 August, 2010

Author Biographies

Hesham H Radwan
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Fars K Alanazi
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Kayyali Chair for Pharmaceutical Industry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Ehab I Taha
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
Hamdy A Dardir

Central Laboratory for Drug and Food Analysis, Ministry of Health, P. O. Box 59082, Riyadh 11525, Saudi Arabia

Ihab M Moussa
Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2460, Riyadh 11451, Saudi Arabia
Ibrahim A Alsarra
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Center of Excellence in Biotechnology Research, King Saud University, P. O. Box 2460, Riyadh 11451, Saudi Arabia
Published
2013-08-10
Section
Articles

Journal Identifiers


eISSN: 1684-5315