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African Journal of Biotechnology

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In vivo extracellular matrix protein expression by human periodontal ligament after stimulation with orthodontic force

José A Morales-González, José Gutiérrez-Salinas, Eduardo Madrigal-Santillán, Jaime Esquivel-Soto, Cesar Esquivel-Chirino, Manuel García Luna y González Rubio, Marco A Becerril-Flores

Abstract


It is well known that the orthodontic force applied to teeth generates a series of events that remodel the periodontal ligament (PDL). Extracellular matrix proteins (ECM) are described as molecular regulators of these events. However, the exact contribution of these proteins in human PDL modeling by orthodontic force application in vivo is not known. The aim of this present study was to evaluate the protein expression of fibronectin, laminin and vitronectin by human PDL from teeth on which orthodontic force was applied. Twenty healthy individuals were included in the study. PDL was obtained from teeth after a 3-week treatment with orthodontic force. PDL-protein samples were separated on 7.5% SDS-PAGE Western blot analysis with specific monoclonal antibodies for fibronectin, laminin and vitronectin. Bands were visualized with an enhanced chemiluminescence detection system and densitometric. Scanning of bands was carried out to compare differences in protein expression. A significant increment in fibronectin (13.9%), laminin (16.5%) and vitronectin (14.2%) expression was found in PDL from teeth treated with orthodontic force for 3 weeks in comparison with teeth in the control group. Our results support the concept that molecular changes take place by application of orthodontic forces to the PDL. Over expression of these proteins suggests that extracellular matrix (ECM) remodeling could be generated in response to mechanical stress.

Keywords: Extracellular matrix proteins, periodontal ligament, orthodontic force

African Journal of Biotechnology Vol. 9(34), pp. 5599-5604, 23 August, 2010



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